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 Wysłany: Śro 8:15, 23 Mar 2011 Temat postu: mbt schoenen Calmodulin immunoelectron microscopy |
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Calmodulin immunoelectron microscopy of colloidal gold labeling
The higher the concentration , the longer duration of action ,[link widoczny dla zalogowanych], adverse effects on the ultrastructure of the more serious ,[link widoczny dla zalogowanych], and the higher concentration of H: O. Also easy to slice a lot of small bubbles formed on the surface , impeding the H: O. Contact with the resin is not conducive to antigen exposure. We use l of a 2% HzOz, for 10 minutes or so , the effect is better. V. , Tritox-100 cells used in the detection of antigen ,[link widoczny dla zalogowanych], in order to make larger antibody molecules and colloidal gold particles into the cell, often need to use Tritox-100 to increase the permeability of cell membranes . It goes without saying , Tritox could undermine the use of a 100 fine structure of cells . Because calmodulin localization is embedded within the cells after staining ,[link widoczny dla zalogowanych], antigen-antibody binding was carried out in ultra-thin sections , there is no increase in membrane permeability problems. Therefore, a cell 100 AR agent Tritox minimize or even eliminate the use of concentration do not have six , rinse the slices in the dyeing process to go through the rinse repeatedly . In the absence of support for film and the use of H0. Reasons, the process of ultrathin sections in the rinse off easily (¨. with the second antibody treatment,[link widoczny dla zalogowanych], inadequate rinsing produce colloidal gold particles of non-specific adsorption , leading it difficult to judge results , rinse and repeat excessively may result in specific Combination of colloidal gold particles are washed off so strong 9 benefits , such as shad feed just rinse is indeed an important factor affect the results . Our approach is , the left hand gripping forceps tweezers rejection network or dropper bottle right hand edge of the drop by drop Slowly washed 2 to 3 minutes is better. '( see insert this map )
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