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 Wysłany: Pią 15:53, 18 Mar 2011 Temat postu: ghd outlet wdn lcr zmh cag |
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Statistics in bioequivalence study of the significance of
Degree of hatching box,[link widoczny dla zalogowanych], after 24h culture, the 2 drugs divided by the peak concentration 0.01,0.1,1.0,10.0,100 g · mL5 drug concentration, the concentration of each compound set up six holes; each block plates, set of tumor cells without drug control and cell-free control, the 6 holes, so that the final volume of each hole 200L; the culture plate and then set incubator,[link widoczny dla zalogowanych], culture 48h. 1.4 absorbance with MITr method, at each hole to join 5mg · mLMTr solution 0.02mL,[link widoczny dla zalogowanych], and then cultured 4h; remove the culture plates after,[link widoczny dla zalogowanych], 2000r · min centrifugation 10min, supernatant was added DMSO0.2mL, home micro oscillator , the full oscillation 5min, measured by ELISA absorbance value (A). Drug inhibition rate (IR) = (A. A Asensitivity / A ... A A.) X100% 1.5 half-inhibitory concentration (inhibitoryconcentration50%, IC50) drug concentrations according to the IR value, the Probit transformation, obtained Ic. . SPSS 1.6 Statistical software used t test, linear correlation test. Received date :2006-05-08 Revised :2006-06-2O Author: Wei Yuning (1972 a), female, competent pharmacists, mainly engaged in clinical pharmacology studies room Corresponding author: Wei Yuning Tel: (010) 66937405E- mail: w4244 @ sina. com · Technology Corner 2 Results 2.1 The two drugs on proliferation of gastric cancer cell lines with different concentrations of Taxol treatment of 5 cell lines, cultured 24h. The results showed that with increased drug concentration, 2 inhibition of gastric cancer cells significantly increased. When paclitaxel concentration /> 0.1g · mL, the survival rate of cells in the treatment group than in the control group significantly (P 0.05); drug concentration of ≤ 1g · mL,[link widoczny dla zalogowanych], the sensitivity of the SGC7901 group was higher than the same concentration inhibited MKN45 susceptibility group ( P 0.1g · mL ~, the SGC7901 susceptibility groups in the same inhibition of the same drug concentration was significantly higher than MKN45 sensitivity group (P <0.05), that the differentiation of gastric cancer cells in the Osage Lee Platinum is more sensitive. 2.2 The half inhibitory concentration results showed that the sensitivity of gastric cancer cell lines, MKN45 and SGC7901 sensitive than on the drug paclitaxel oxaliplatin, 2 were equally sensitive to paclitaxel; and in the differentiation of SGC7901 of paclitaxel, oxaliplatin sensitivity was significantly higher than MKN45. 3 advanced gastric cancer discuss the effective rate of paclitaxel of 15% to 24%. Oxaliplatin is a 3rd generation platinum anticancer drugs, the first for the treatment of colorectal cancer clinic. In recent years, found that the treatment of gastric cancer highlights. In this study, gastric cancer cells were found in 2 of oxaliplatin-sensitive; but the result was worse than that of paclitaxel. The results showed that the degree of differentiation of gastric cancer cells to paclitaxel, oxaliplatin different sensitivities. This is consistent with those reported.
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