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<table border="0" cellpadding="3" cellspacing="1" width="100%" class="forumline"> <tr> <th class="thCornerL" width="22%" height="26">Autor</th> <th class="thCornerR">Wiadomość</th> </tr> <tr> <td width="22%" align="left" valign="top" class="row1"><span class="name"><a name=""></a><b>yxgaxjlywf</b></span></td> <td class="row1" height="28" valign="top"><table width="100%" border="0" cellspacing="0" cellpadding="0"> <tr> <td width="100%"><img src="http://picsrv.fora.pl/xeon/images/icon_minipost.gif" width="12" height="9" alt="Post" title="Post" border="0" /><span class="postdetails">Wysłany: Sob 22:41, 12 Mar 2011<span class="gen"> </span> Temat postu: nhm psk hyj pdr</span></td> </tr> <tr> <td colspan="2"><hr /></td> </tr> <tr> <td colspan="2"><span class="postbody">Elderly patients with pulmonary heart disease endogenous digitalis substances and the determination of atrial natriuretic peptide ... <br /> <br /> <br />atogs & derivatives {atrialnatriureticfactor; aged that the endogenous digitalis-factor of the current (EDLS) and atrial natriuretic factor (ANF) is involved in the regulation of cardiac function in vivo an important vasoactive substances EDLS of plasma Cor-reported, and views differ. J. To investigate the EDIS and the ANF in the elderly in the pathophysiology of pulmonary heart disease and changes in the role of clinical significance. The experimental method for simultaneous determination of the radioimmunoassay of 30 cases of elderly patients with acute pulmonary heart disease and remission of the Unit 1 Division One South Building, General Hospital of PLA, Beijing 1008532 Department of PLA General Hospital, South Building of the kidneys when EDLS and ANF plasma levels, and with 25 healthy elderly were compared. 1 objects and methods I. 1.1.1 Object Group 1 3O patients with pulmonary heart disease living in our hospital in patients with pulmonary heart disease in elderly men, average age 67 years (60 to 78 years), basic causes are chronic bronchitis, emphysema diagnosis of pulmonary heart disease standards in accordance with the 1977 second national professional meetings Cor revised diagnostic criteria, according to criteria of the disease and efficacy of classification is divided into acute and remission. When the acute attack are associated with different degrees of t2, dysfunction, as one function by NYHA classification method, Ⅱ grade 1l down, Ⅱ grade 13 cases, Ⅳ grade in 6 cases. All patients had no history of hypertension and coronary heart disease. ECG and biochemical tests of liver and kidney function values ​​were within the normal range, were not taken within 1 week before the experiment had digitalis drugs. 1.1.2 After a healthy elderly group of history taking, physical examination, liver and renal function biochemical tests, chest X-ray, ECG, echocardiography confirmed 25 cases of healthy elderly men. The average age of 64.6 years (60 to 74 years). 1.2 Methods 1.2.1 Collection and handling of blood samples fasting state early in the morning to take a quiet cubital vein 5ml, containing EDTA and placed in a test tube enzyme inhibitory peptide, mix, 4C3000r/min centrifuged 10rain. Remove the plasma, releasing a batch tested with 30C in the refrigerator. And simultaneous determination of arterial blood gas and liver and kidney biochemistry. 1.2.2 Determination of plasma concentrations of ANF and EDLS both companies to use northern China isotopes Institute of atrial natriuretic peptide immunoassay RIA kit and digoxin radioimmunoassay kit. Determination of plasma ANF in strict accordance with the instructions of the steps. Determination of plasma EDLS is digoxin radioimmunoassay determination kit, make the following improvements in the methods: improving their sensitivity. The antibody titers raised to 1:6000, antiserum mixture, the first in the 37 ℃ for 0.5h, and then added a digoxin EDLS I and II anti-separation agent again after the separation and determination 37C0.5h incubation. The method of determination of the range of 62.5 ~ 2000pg/ml, sensitivity was 17pg/ml, coefficient of variation 005) a linear correlation analysis in Table l2.2 see Table 2. Table 1 Comparison of plasma ANF Wang Shi EDF and a p / rtg · m1) Table 2 Plasma EDF, ANF, arterial blood gases and cardiac function in the correlation coefficient between grade to 30) related to the number (rjEDFAF3 discussion in recent years studies have shown that some vasoactive substances in pulmonary heart disease and prevention have an important role. EDLS is a powerful cardiotonic, diuretic and natriuretic vasoconstriction can compete with the plot of Pakistan due to receptor binding, NakATP enzyme inhibition in the radioimmunoassay of digoxin antibody with cross reaction. ANF is a strong heart, natriuretic, vasodilator, and blood pressure lowering effect of peptides Attack of the EDF and ANF plasma levels were significantly increased. EDLS and ANF in plasma prompted a change in the pathophysiology of pulmonary heart disease. Many research data shows that many can increase the cardiac load</span></td> </tr> </table></td> </tr> <tr> <td colspan="2" height="1" class="spaceRow"><img src="http://picsrv.fora.pl/xeon/images/spacer.gif" alt="" width="1" height="1" /></td> </tr> </table>
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