 Wysłany: Czw 4:27, 17 Mar 2011 Temat postu: UGG boots Italia rok toz wli ajr |
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| Novel bcl-2 antisense oligonucleotide F951 induced apoptosis in human leukemia HL60
18 (10) :1781-7ApoptosisofHL60cellsinducedbyF951,UGG boots Italia, anovelbcl-2geneantisense0lig0de0xynuele0tideLIDong-liang, LOLian-huang, LINZhen-xing, CHENYing-yu (FujianInstituteofHematology, theAffiliatedUnionHospital, FujianMedicalUniversity, Fttzhou350001,belstaff outlet, China) Abstract: AimToobservetheapoptosisofhumanleukemicceHsinducedby51. anovelbcl-2anti-senseoligodeoxynucleotide. MethotisHL60cellswereculturedwith51invariantdoses. Apoptoticcellsweredetectedbyflowcytometry. DNAladder. e-lectronmicroscopeobservationandtermina1. . deoxynu. . cleotidyltransferasemediatednickendlabeling (TUNEL). ResultsAfterHL60cellsweretreatedfor48hours, mitochondriaapoptoticcellscanbedetectedattheratioof3.00% intheuntreatedgroup. 13.57% intheFNScontrolgroupand30.95%, 38.08%. 52.55% th5, 10,20 m01. L51respective-ly; theratioofcellswithcaspaseactivitywas0.08% intheuntreatedgroup. 0.14% in Mount eFNScontrolgroupand43.68%,mbt scarpe outlet, 60.54%,tory burch flats, 80.37% with5,Christian Louboutin Ireland, 10,20 p ~ mol · L ~ F951respectively. TypicalDNAladderwasseenfromgelelectrophoresisinF951treatedgroups, andmoreapparenteffectintheaspectofindu-cingDNAladderhadbeenobservedwiththeimprove-mentofF951concentration. DetectedthroughTUNELandelectronmicroscope, apoptoticcellsinuntreatedgroupandFNScontrolgroupcanonlybefoundbychance, butverycommonlyseeninF951treatedgroupsandmorefrequentlywiththeimprovementofF951concentration. Conclusion51caninducecellapoptosisinHL60cells. Sucheffectisachievedthroughtheinhibitionofbcl-2geneexpressionbyF951whichinitializeapoptosispassagewayinconsequence. Keywords: leukaemia; genes; bcl A 2; oligodeoxynucleotides; antisense |
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