 Wysłany: Nie 17:40, 27 Mar 2011 Temat postu: Factor Ⅷ during the preparation of the FFP by tim |
|
| Factor Ⅷ during the preparation of the FFP by time and temperature
001) Keywords: fresh frozen plasma (vvP); F Ⅶ: c; temperature; time Key words: R446.11 Document code: A Article ID :1006-7655 {2001) 01-O82-01 was 』- Discussion prepared under different conditions and storage of plasma on the F Ⅷ: C activity, the author takes a different time and temperature conditions, tests, the results reported below. 1 Materials and Methods 1.1 Instruments SYSMEXcA a 50 coagulometer, Iabsysten ~ pipette 5-4Oral, 40 ~ 200ta. 1.2 Reagents DADEBEHRING kit (buffer waves, lack of F Ⅷ: c in plasma, AP Ji reagent), O. O25m ~ | lLcaC solution. 1.3 Methods The plasma 5ta + 45 + lack of buffer F Ⅷ: c plasma heat 1 minute + Am 5l reagents 5ota heat 3 minutes + CaCl ~ solution 50vl detection (heat, time control, detection of blood coagulation analyzer CA-50 on.) Sample source and treatment: physical examination and blood tests by the Ministry of Health sets standards for operator's unpaid blood donors who were blood 1O; per cent of blood collected will be divided into plasma, were installed in 13 sub-tubes (each about 0. 5m1), 1 支 × 10 people were tested immediately, the other set to 32 ℃ (3 × 10 servings), 22oC (3 × 10 servings), 4 ℃ (3 × 10 people), frozen into blocks of a post-5O ℃ Save for a 20 ~ C (3 × 10 people), for 6 hours, 12 hours, 24 hours plasma in F Ⅷ: c activity. 2 under different conditions the results of plasma F Ⅷ: c activity test Annex. Immediately detects F Ⅷ: C activity. 50 ~ C after frozen into blocks. 20 ℃ for 6 hours, 12 hours, 24 hours, 4oC6 hours, 22oC6 hours of testing F Ⅷ: C activity was no significant difference (P> 0.05). And 32oC6 hours, 12 hours, 24 hours, 22 ℃ 12 hours, 24 hours, 4 ~ C12 hours, 24 hours testing F Ⅷ: C activity difference was significant (P (O.05). So as to ensure that fresh frozen plasma F Ⅷ: C activity, preparation should be completed within 6 hours, and frozen into ice. with samples down wrapped 10 times at different temperatures under FVJ: C activity in c% to; car threshold table data for the blood instantly white stems I Fruit Jing points than the 3 to discuss preparation of fresh frozen plasma (FJ), to maintain the F Ⅷ: c activity, not only in the collection of blood on the requirements of a smooth, smooth,tory burch flats, in the preparation process at different temperatures and time to ask on plasma F Ⅷ: C activity have a greater impact, preparation process temperature is not high .32 ~ C6-hour activity to the loss of 52.4%. time is not long at room temperature for 24 hours on the loss of 70%. With the popularity of voluntary blood donation and blood components to promote MAP red blood cells, are often not timely preparation of ingredients , resulting in F Ⅷ: C activity lost. Therefore, I believe that preparation of fresh frozen plasma, should be at room temperature, requires from the beginning to the preparation of blood plasma into the ice time is not more than 6 hours |
|
