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 Wysłany: Wto 8:45, 01 Mar 2011 Temat postu: 莪术、三氧 |
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莪术、三氧化二砷对晶状体上皮细胞增殖及信号转导分子的影响
FujianCollegeofTraditionalChineseMedicine，350003，China)[Abstract]Objective：ToinvestigatetheeffectofRhizomaCurcumae(RC)，arsenitetrioxide(As2O3)onproliferationariasignaltransductionmoleculeinlensepithelialcell(LEC)，inordertoprovideexperimentevidenceforpreventionandtreatmentofaftercataract．Method：ProliferationofculturedbovineLECwereinducedbyinducedbyrecombinanthumanbasicfibroblastgrowthfactor(rhbFGF)；InhibitoryratesofLECproliferationinducedbyRC，As2O3weredetectedbymethylthiazolyltetrasolium(MTY)；Inhibi-toryeffectsofexpressionofproliferatingcellnuclearantigen(PCNA)inducedbyRC，As2O3inLECwereassayedviaflowcytometer(FCM)；ConcentrationsofLECcalcium([Ca2]i)weredeterminedbyspeetrofluoremeter，intracellularconcentrationsofcyclica．denosinemonophosphate(cAMP)andcyclicguanosinemonophosphate(cGMP)ofLECweremeasuredbyradioimmunoassay．Re-stilt：InhibitoryratesofRC，As203onLECproliferationinducedbyrhbFGFincreasedsignificantly，showingdose—dependent(P<0．01)．PCNAexpressionofLECproliferationinducedbyrhbFGFweredownregulatedobviouslybyRC，As2O3，showingdose—de—pendent(P<0．01)．Concentrationsof[Ca2]．andcAMPincreasedandcGMPdecreasedsignificantlyinLECofproliferationinhibi—tedbyRC，As2O3(P<0．01)．Conclusion：RC，As203CaninhibitLECproliferationobviously．Signaltransductionsof[ca]i，cAMP，cGMPmaybetheimportantmolecularmechanism．TherearebroadprospectforRC，As203onpreventionandtreatmentofaf-tercataract．[Keywords]RhizomaCurcumae；arsenitetride；lensepithelialcell；proliferation；sis,~transduction[责任编辑刘观]·728·
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