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Dołączył: 03 Mar 2011
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 Wysłany: Pią 18:57, 25 Mar 2011 Temat postu: All-trans retinoic acid on the SHI-1 cell line gly |
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All-trans retinoic acid on the SHI-1 cell line glycosyltransferase expression
Of a Tl,, rr3, T4. Glycobiology research has revealed , O- glycan synthesis in the initial reaction of Tl,, rr3 first UDP-GMNAc catalyzed the transfer reaction times by the T4, rI7 such as catalytic transfer of the follow-up of the UDP-GalNAc . This experiment revealed the first UDP-GalNAc transfer reactions , subsequent reactions mainly SHI-1 to T4 . PP. GalNAc. T1 , and in most human tissues are widely distributed. However , PP. GalNAc. T3 found mainly in the human testis , kidney, gastrointestinal and reproductive systems. PP-GalNAc. T4 mainly expressed in stomach and colon with high and low expression of the small intestine , urinary genital and lung 4J. Currently, only Tl , and are reported in the normal expression of white blood cells , rr3, T4 has not been reported expressed in the normal white blood cells . Our experiments showed that the leukemia cell line SHI. 1, not only PP-GalNAc-T1, T2 expression , but also rr3, T4 expression, and the T4 expression is relatively high. Therefore, in the white blood cells , PP-G ~ NAcT3, T4 may only be expressed in leukemia cell lines . In addition experiments show that , SHI. 1 cells expressing O-GnT, 133GnT1,[link widoczny dla zalogowanych], 133GnT5, the expression of which 133GnT5 than O-GnT, 133GnT1 high. As for the glycosyltransferase in the infiltration of leukemic cell invasion in the role will be explored in future studies . Prior to joining inducing agent all-trans retinoic acid effect , the different glycosyltransferase expression of different changes. Just as described in the results , ppGMNAcT1, T2, T3, T4, O-GnT, 133GnT5, 133GnT1 of all-trans retinoic acid response is not exactly the same way , these indicate that the induction of all-trans retinoic acid , different Glycosyltransferase expression of different, how different glycosyltransferases on the all-trans retinoic acid in the reaction mechanism. But the most elevated expression , indicating that the differentiation agent ATRA induction after joining , SHI-1 cells will be increased glycosylation . Cause of this phenomenon remains unclear , needs further study . [
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