Abercrombie france,Clustering of hepatitis B virus

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Clustering of hepatitis B virus infection markers within the family

Key words , hepatitis B virus; familial aggregation; disease susceptibility
】【Abstract AIM: To determine the spread of HBV virological profile of Chinese clustering infected families with threegeneration genetic information. METHODS: All the patients who referred to our department were questioned for HBV family history from 2002 to 2005. For those families with threegeneration or more genetic information, their blood were drawn after signed informconsents were obtained and tested for hepatitis B surface antigen (HBsAg), antibody to HBsAg (antiHBs), hepatitis Be antigen (HBeAg), antibody to HBe (antiHBe) and antibody to hepatitis B core antigen (antiHBc) using commercially available enzymelinked immunosorbent assay (ELISA). HBV genome copies in serum were tested by realtime quantitive PCR. The first member of a family identified as a virus carrier was considered an index case. All study subjects were asked to fill out a questionnaire designed by Epiinfo software on general data, history, results of serologic analysis, and kinship to the index case. All the data of each subject was input into computer for statistic analysis. RESULTS: A total of 237 serum samples of 27 familes were tested. Out of 237 index cases and household contact subjects, 45.6%,[link widoczny dla zalogowanych], 5.9%, 26.7%, 11.8%, 62.9%, 28.3% subjects were identified HBsAg, antiHBs, HBeAg, antiHBe, antiHBc, HBV DNA positive respectively. The patients with positive HBV DNA, HBeAg or HBsAg were predominantly male and the mumber peaked at the age of 18 and 45 years old. CONCLUSION: The seroprevalence of HBV markers in clustering infected family is different from that in general population; the infected individuals have a natural course of chronic HBV infection and mostly are infected as they are infants, which is probably associated with host genetic susceptibility to HBV. 【Keywords】 hepatitis B virus; family clustering ; disease susceptibility
Abstract Objective: To study HBV markers and HBV DNA in serum in the clustering of infection within the family of the popular model. Methods: 2002/2005 treatment of infectious diseases in our hospital from the remote mountain areas positive for HBsAg proband, inquired of his family history, screening with three or more genetic information on behalf of the family. signed the informed consent form, this family all the people in the epidemiological investigation,[link widoczny dla zalogowanych], collection of blood samples. detected by ELISA HBsAg, anti-HBs, HBeAg ,[link widoczny dla zalogowanych], anti-HBe, anti-HBc; by RealTime PCR quantitative detection of HBV DNA. Results: A total of 27 families and 237; HBsAg, anti-HBs, HBeAg, anti-HBe, anti-HBc, HBV DNA of the overall positive rates were 45.6%, 5.9 %, 26.7%, 11.8%, 62.9%, 28.3%; in the clustering of HBV infection in the family, male HBsAg, HBeAg, HBV DNA positive rate was significantly higher than women; HBsAg and HBV DNA into a bimodal distribution of the relative age of the main concentrated in the 18-year-old and 45 years of age. Conclusion: The clustering of infection in the family,[link widoczny dla zalogowanych], HBV epidemiological data has its own characteristics; familial aggregation of chronic HBV infection consistent with the natural course of infection, mostly infants and young children of the infection, may be related to host genetic susceptibility. Key words hepatitis B virus; familial aggregation; disease susceptibility
0 Introduction
China is a high incidence of HBV . Early HBV infection was found familial aggregation, rates of chronic family members, primary liver cancer risk,[link widoczny dla zalogowanych], response to antiviral therapy, the response of HBV vaccine were associated with the general population there is a big difference, suggesting that these families have a specific genetic background [1]. To this end we study of serum HBV markers and HBV DNA in the aggregation of the prevalence of infection within the family model, are reported as follows.
1 objects and methods
1.1 object 200201/200512 treatment of infectious diseases in our hospital from the remote mountain HBV infection as the proband (index cases), by asking to know their family history, initial screening can provide the genetic information of more than three generations information on the family, informed consent. investigators unified epidemiology training, with Epiinfo 3.32 (www.cdc.gov / epiinfo) software to make epidemiological survey forms. survey included general condition, HBV infection, clinical history , drinking history, family history and relatives of the proband with the relationship. EDTANa2 anticoagulant in peripheral blood specimens from each of 10mL, centrifugation (> 2000 g, 10 min) serum was separated, set -20 ℃ cryopreservation standby. 1.2 Methods ELISA assay HBsAg, anti-HBs, HBeAg, anti-HBe, anti-HBc IgM and anti-HBc IgG, commercial kits purchased from 3v biotechnology company. HBV DNA quantification by RealTime PCR method, apparatus is ABI 7300, the 1 × 103,1 × 104,1 × 105,1 × 106,1 × 107,1 × 108 copies / mL of the standard curve to establish standards, testing is greater than 1 × 103 copies / mL is defined as HBV DNA positive. more uniform testing of each item by hand.
Statistical analysis: All data from 2 people at a time Epiinfo input, respectively, consistent with the results after testing all the data into SPSS 11.5, compared with the χ2 test rate.
2 results
2.1 survey of 27 families were collected, the Department of 23 people, minimum 4 families, a total of 237 (male 123, female 114) people. Proband 27, the proband first, second and third, respectively, blood is 102,41,21 people, non-genetically proband (spouse and blood relatives of the spouse) family members 46. HBV markers distribution in different age groups in Table 1.
Table 1HBV serum markers and HBV DNA distribution in different age groups (omitted)
2.2HBsAg popular features of 108 HBsAg-positive patients (45.6%), mainly in the 18-year-old and 45-year-old two age peaks, accounting for 70%, in addition, 0 to 5 age group there are still a small peak (Figure 1); male positive rate (52.0%) was significantly higher than women (38.6%), P = 0.026. anti-HBs total of 14 patients (male / female = 9 / 5), only 5.9%.

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